http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021052124-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_626666c6c8665b8b3057be1fa0641270 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2020-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b21b12e6bd18262ee4b62b3b820980e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7a18f46eecc8c2078515683d60fa716 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e29d35502b00910e79024cee2430374 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d5d8d49f7d327a860665f082be31495 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35f214dde91e6989037a808a9ee001b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9810b609e788dd6300c3e815b7467273 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_304544fba459e4e97b3001146159b735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31129f44ed0f17dd003b05ada98ffe16 |
| publicationDate | 2021-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2021052124-A1 |
| titleOfInvention | Preparation method for rna library, sequencing method and kit |
| abstract | The present invention provides a preparation method for an RNA library, a sequencing method and a kit. Disclosed in the present invention is a method for preparing an RNA library. The method comprises: generating first strand cDNA by means of inverse transcription by using RNA as a template, to obtain an RNA/cDNA hybrid; and adding a first preset sequence to at least one end of the RNA/cDNA hybrid to form an RNA/DNA hybrid, the RNA/cDNA hybrid being an RNA library, and the first preset sequence being located on at least one end of a cDNA strand. This method is simple and quick to prepare an RNA library, and the constructed RNA library can be directly used for sequencing. Because this method has no second-strand cDNA synthesis process, that is, no cDNA amplification and enrichment process, bias and errors caused by PCR amplification and enrichment can be avoided. In addition, disclosed in the present invention is an RNA sequencing method, for directly determining cDNA sequence information to obtain RNA sequence information. Also disclosed in the present invention is a kit for RNA sequencing. This kit can be utilized to construct an RNA library and used for the sequencing of the RNA library. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113718023-A |
| priorityDate | 2019-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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