http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021049960-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_188f17ee54c53d14cbb7a521fcbd9222 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-21001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-465 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
| filingDate | 2020-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3a0087b0c29919e6ea05281d6983835 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ee7c1d8424ecf6914aee6f9c2d328fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2db64710f05bae566a8221053c285677 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90405a8923deaab1c60155c129c7fedf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e390f20757122a7935a67959862a4980 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1de1794f073b8e3bf1929a760224e731 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69671207c81adac3118ee10907224140 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02da5611d7af4061b4e2d7485b793041 |
| publicationDate | 2021-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2021049960-A1 |
| titleOfInvention | A new, non-specific thermolabile nuclease active at low temperature, in wide ph range and high concentration in salts |
| abstract | The subject matter of this invention is a new thermolabile PPR nuclease or its enzymatically active fragment exhibiting high catalyst activity in difficult reaction conditions (particularly high concentrations of salts and other additives commonly used in the processes of the purification of proteins and viruses, low temperatures and wide range of pH), where the nuclease sequence is SEQ.2 or a sequence that shares at least 40% of its identity. The subject matter of the invention is also the gene encoding PPR nuclease or its enzymatically active fragment; a particle of nucleic acid encoding the PPR nuclease or its enzymatically active fragment; expression plasmid including the sequence of the PPR encoding gene; recombinant strains of Escherichia coli JM109(DE3) pD454-PPR-AmpR and E. coli ArcticExpress (DE3) pD454-PPR-AmpR; the method of PPR nuclease protein production, application of PPR nuclease in processes of purification of recombinant proteins of significantly lower DNA content. As well as for decontamination of reactive reagents and mixtures for PCR, qPCR, RT- PCR, RT-qPCR, RCA, LAMP and NGS in order to obtain higher sensitivity and specificity of relevant genetic analyses; application of the PPR nuclease in processes of virus vector purification (particularly lentiviruses [LV], adenoviruses [AV, AAV] and retroviruses [RV] used in modem gene and cell therapies (chimeric antigen receptor [CAR] T-cell immunotherapy)); application of the PPR nuclease in exosome purification processes for therapeutic or diagnostic purposes; application of the PPR nuclease in processes of recombinant protein purification, particularly enzymes, antibodies, vaccination antigens, products used in cell therapies and other therapeutic proteins. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022248874-A1 |
| priorityDate | 2019-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.