| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff8b7be91f9f7275874fd80bd4141923 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-185
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-01 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-99003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-05002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01274
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01119
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01118
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01215
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-99004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01121
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01012 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-185 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate |
2020-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f897bdf455173b5f6e4b04db987615fa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a1d4722e8f3f69fb740362661149d97
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f2dac919c4a93c391e1cd17c025e00f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2bb9fcca0726e264ebad085f057249b7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd780297056462afd8f8c5bb44396ad5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c05920d8b0d213ecc04836053d81bed5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e57db0be387cbe786690bf4f5a08645d |
| publicationDate |
2021-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2021036788-A1 |
| titleOfInvention |
2-keto-l-gulonic acid production strain and construction method therefor |
| abstract |
Provided is a construction method for a 2-keto-L-gulonic acid production strain, comprising one or more of the following steps in any order: 1) interrupting a membrane-bound glucose dehydrogenase encoding gene in the chromosome of Tatumella; 2) interrupting a glucose kinase encoding gene; 3) interrupting a gluconate kinase encoding gene; 4) interrupting a 2-ketose or aldose reductase encoding gene and/or an isozyme encoding gene thereof; 5) recombinantly expressing an NAD(P)+ type glucose dehydrogenase; 6) recombinantly expressing an NAD(P)H type 2,5-diketo-D-gluconate reductase; 7) overexpressing 2,5-diketo-D-gluconate endo-transporters ARU92990 and ARU92994; and 8) interrupting a PTS component encoding gene in the chromosome of a cell to obtain genetically engineered bacteria. The 2-keto-L-gulonic acid production bacteria constructed by said method can produce 2-keto-L-gulonic acid by using glucose as the raw material by using a one-step fermentation process or whole cell catalysis. |
| priorityDate |
2019-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |