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publicationDate 2021-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2021034974-A1
titleOfInvention Methods and compositions for tracking nucleic acid fragment origin for nucleic acid sequencing
abstract The present disclosure provides methods and compositions for tracking nucleic acid fragment origin by target-specific barcode tagging when original nucleic acid targets break into small fragments. Nucleic acid targets are captured in vitro on a solid support with clonally localized nucleic acid barcode templates. Many nucleic acid targets can be processed simultaneously in a massively parallel fashion without partition. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing in order to accurately identify genomic variants, haplotype phasing and assembly, for example.
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