http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021027866-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_08dde60e3e0ca1e6ffaa5143e5c8477a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6841 |
| filingDate | 2020-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69bca18f00b857bb6f107a3c8547a50b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c00c038b97a926152d89517a5341c948 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fd00cca962b08f8fc5b06853715e312 |
| publicationDate | 2021-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2021027866-A1 |
| titleOfInvention | Rna in-situ hybridization optimization method used for cucumber seedling gene mapping research |
| abstract | Provided is an RNA in-situ hybridization optimization method used for cucumber seedling gene mapping research. First, on the basis of characteristics of cucumber seedling plant material, a method for plant cultivation, sampling, and fixing is improved, thereby obtaining a complete plant structure containing a target tissue. Next, operating means such as slice preparation, hybridization pre-processing, hybridization incubation and slice sealing, and color reaction microscopy are optimized in a targeted manner, thereby improving the reliability, accuracy, and specificity of a gene mapping result. Finally, a processing solution suitable for after termination of color reaction in cucumber seedling mature tissue is designed in a targeted manner, facilitating elimination of interference from cell inclusions, and direct observation of the distribution of hybridization signals. |
| priorityDate | 2019-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 104.