| abstract |
The invention relates to a method for purifying a target substance starting from a fluid to be treated which comprises at least one impurity, said method comprising the following steps: – provision of a stream of the fluid to be treated; – treatment of the stream of fluid to be treated using a chromatography step in a first separation unit (1); – collection of a fraction enriched with the target substance, in a first tank (4); – viral inactivation of the fraction enriched with the target substance, said viral inactivation comprising: passing the fraction enriched with the target substance collected in the first tank (1) through a second separation unit (2), via a bypass line (9); passing a viral inactivation solution through the second separation unit (2), via the bypass line (9); mixing the fraction enriched with the target substance and the viral inactivation solution; and collecting the mixture of the fraction enriched with the target substance and the viral inactivation solution, in the second tank (5); so as to obtain a fraction depleted of active virus; – treatment of the fraction depleted of active virus using a chromatography step in the second separation unit (2); and – collection of a fraction more enriched with the target substance, at the outlet of the second separation unit (2). |