| abstract |
The present invention relates to a transgenic cloned pig for xenotransplantation in which porcine endogenous retrovirus (PERV) EnvC is negative, α1,3-galactosyltransferase (GGTA1), CMP-N-acetylneuraminic acid hydroxylase (CMAH), isoglobotrihexosylceramide synthase (iGb3s), and beta-1,4-N-acetyl-galactosaminyl transferase2 (β4GalNT2) are knocked out, and human CD46 and thrombomodulin (TBM) genes are expressed, and to a method of preparing the transgenic cloned pig. The transgenic cloned pig according to the present invention can overcome hyperacute and antigen-antibody mediated immune rejection, immune rejection due to blood coagulation, and immune rejection due to complement activity, without causing transfer of porcine endogenous retrovirus that occurs in xenotransplantation. Therefore, the transgenic cloned pig according to the present invention can be usefully utilized as a donor animal for xenotransplantation of organs and cells. |