Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e00d0c1e9270bf172de0c789213f5a6 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14151 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10343 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10351 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-16043 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-16051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2750-14152 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B35-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1037 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86 |
filingDate |
2020-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db0bd22b960c7955c4c1cfec5ffa2032 |
publicationDate |
2021-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2021003374-A2 |
titleOfInvention |
Host systems comprising inhibitors of a gene-editing protein for production of viral vectors |
abstract |
The present invention provides a method of manufacturing vectors containing a heterologous gene-editing protein comprising providing (a) transforming a host system with a nucleic acid cassette containing a promoter operably linked to a gene encoding a gene-editing protein, wherein the host system also contains a heterologous inhibitor for the gene-editing protein, (b) incubating the host system for a time sufficient for vector production and to release the recombinant vector, and (c) recovering the recombinant vector. Also provided herein are cell lines for expressing vectors containing a gene-editing protein with an inhibitor of the gene-editing protein to prevent leaky expression of the gene-editing protein comprising constitutive expression of an inhibitor of a gene-editing protein. |
priorityDate |
2019-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |