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filingDate 2020-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2020259265-A1
titleOfInvention Method for establishing characteristic map of total immune cells in lung of acute lung injury mouse
abstract Provided is a method for establishing a characteristic map of total immune cells in the lung of an acute lung injury mouse, wherein the method comprises the steps of: extracting total immune cells in the lung of a mouse; marking an antibody for flow mass spectrometry; dyeing the immune cells with the antibody; and performing flow mass spectrometry analysis. On the basis that cell purity is ensured, the total immune cells in the lung of the mouse that have a high yield can be separated; the yield of the separated total immune cells in the lung of the mouse is higher than that of a grinding method, and the cell viability thereof is greater than that of the traditional grinding method. In the establishment method, the connection of a metal isotope and an antibody is stabilized by means of using a kit, and a flow mass spectrometry channel is designed on the basis of a channel interference minimization principle, realizing comprehensive description on classification and functions of the total immune cells in the lung of the mouse, thereby systematic detection and classification on the total immune cells in the lung of the mouse can be carried out, and at most 43 markers can be detected simultaneously; and dynamic changes of the total immune cells in the lung of the mouse can be observed.
priorityDate 2019-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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