| abstract |
Disclosed are a KASP labeled primer for detecting a high molecular weight glutenin subunit Dy10-m619SN of the wheat, and an application thereof. The KASP labeled primer comprises a forward primer F1, a forward primer F2, and a reverse primer R1; or comprises a forward primer F1, a forward primer F2, and a reverse primer R2, wherein sequences of F1, F2, R1, and R2 are as represented by SEQ ID NO. 1-SEQ ID NO. 4. The molecular labeled primer provided by the present invention can be used for identifying an SNP mutation site of a subunit Dy10 to be detected of the wheat, can determine the phenotype of the high molecular weight glutenin subunit Dy10-m619SN of the wheat according to the SNP mutation site or is used in combination with an SDS-PAGE technology to detect the phenotype of the subunit to achieve the purpose of assisting in identifying the wheat to be detected. |