| abstract |
Provided are a clinical-grade autologous bronchial basal cell, a transfusion formulation thereof, and a preparation process. The preparation process comprises the following steps: extracting in vitro active bronchial brush biopsy tissue for digestive treatment, and collecting cells after digestion is terminated; performing plating culture on the digested cells by using a culture plate having trophoblast cells plated thereon, collecting cells, performing amplification culture on the digested cells by using a culture plate having trophoblast cells plated thereon, and when cells grow to cover 50%-90% of the surface area of the culture plate, performing secondary culture; and when secondary culture cells grow to cover 85%-95% of the surface area of a culture dish, digesting and collecting adhered cells, and washing. The prepared bronchial basal cell can be used for repairing damaged lung tissue. |