| abstract |
Disclosed are a detection chip (100) and a usage method therefor, and a reaction system. The detection chip (100) comprises a first substrate (10), a microcavity defining layer (11), a hydrophilic layer (14) and a hydrophobic layer (13). The microcavity defining layer (11) is located on the first substrate (10), and defines a plurality of micro reaction chambers (110). Each of the plurality of micro reaction chambers (110) comprises a reaction trap, and the reaction trap comprises a side wall (1101) and a bottom portion (1102). The microcavity defining layer (11) comprises a spacer area (111) located between the plurality of micro reaction chambers (110), and the spacer area (111) comprises a first area (111a) adjacent to the side wall (1101) and a second area (111b) not adjacent to the side wall (1101). The hydrophilic layer (14) covers the side wall (1101) and the bottom portion (1102) of each of the plurality of micro reaction chambers (110), and the hydrophobic layer (13) covers the second area (111b). The detection chip (100) is conducive to enabling liquid drops to automatically enter the micro reaction chambers (110), does not require an external heating device, and is high in integration level. |