http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020182768-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9322bafd839eb1dca8b7566c74612261 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P17-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K36-064 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K36-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P17-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L33-105 |
| filingDate | 2020-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffcd385d8d4f7ff9e3edcfcb0b574ceb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_38e68ff19fba203dd845ef2775bf3085 |
| publicationDate | 2020-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020182768-A1 |
| titleOfInvention | Methods for bioactive lectin production with improved yield |
| abstract | Methods for the production of recombinant lectin proteins in yeast are provided. The methods comprise transforming a Pichia pastoris yeast culture with a nucleic acid expression vector that comprises a gene encoding a PHA polypeptide, wherein the PHA gene is fused to a nucleic acid sequence that encodes a Saccharomyces α-factor prepro- peptide, and wherein the expression of the PHA polypeptide is under the control of an inducible promoter present in the expression vector; maintaining the yeast culture under conditions that support expression of the PHA polypeptide; and isolating the recombinant tetrameric PHA protein from the yeast culture. The methods are suitable for production of recombinant phytohaemagglutinin (PHA) lectin production, particularly PHA-L and PHA-E. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113603772-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113603772-A |
| priorityDate | 2019-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 153.