http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020171949-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a99a14f2bb34497327c34348d1b74386 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2020-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f71ed822f2eea63ab3c43785fd33b6a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de8d1afc4f04cf98b181b5fe01c1a46f |
| publicationDate | 2020-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020171949-A1 |
| titleOfInvention | Multiplex nucleic acid amplification and library preparation |
| abstract | The present teachings relate to the use of multiplex PCR amplification to enrich multiple targets of interest while reducing post-amplification by-products via controlled primer digestion for use during the preparation of massively parallel next-generation DNA sequencing library construction. In one aspect, the present teachings relate to specific primer design, and in another aspect, the present teachings relate to an overall optimization of workflow that increases the efficiency of next-generation sequencing by targeting only user-defined regions of interest and substantially reducing or eliminating contaminating genomic DNA, and other interfering by-products of targeted amplification or unused reactants. Particularly, processes and kits are disclosed for preparing a plurality of multiplex amplification products for targeted next generation-sequencing providing reduced background noise. |
| priorityDate | 2019-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 157.