http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020168061-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_828b259b709f4cda763aaa70d846e4a4 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-33 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
| filingDate | 2020-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b09bc3833a59ee66ba8a21dc0a67a65c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fb03139362bb771f25004d39d05ad2d |
| publicationDate | 2020-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020168061-A1 |
| titleOfInvention | Compositions and methods for protease screening |
| abstract | Compositions and methods are described that provide for screening the activity of genetically modified proteases to identify modified substrate specificity. A series of recombinant peptide substrates are generated that incorporate both portions of a parent substrate peptide (i.e. a peptide substrate of the native or wild-type protease) and portions of a target substrate peptide (i.e. a desired peptide substrate of the recombinant protease). Recombinant proteases are initially screened against a recombinant peptide substrate with a relatively high ratio of parent substrate content to target substrate content. Recombinant proteases identified in the initial screening can be further screened using a different recombinant peptide substrates with a lower ratio of parent substrate content to target substrate content. The screening process can be repeated using recombinant peptide substrates with progressively lower parent substrate content until a recombinant protease is identified with the desired activity and/or specificity towards the target substrate. Recombinant peptide substrates can include identifiable tags, immobilizing/affinity tags, and other features that facilitate detection of protease activity. |
| priorityDate | 2019-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.