http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020157441-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e2d6c63af05648054f8699926d62bcce http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37f4922dfb7777b019e504b885211b8e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-726 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-4719 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 |
| filingDate | 2020-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_863eb839421beb83df4068baa9773c29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_825b81456cfb9f55b67fc210543d67a6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c1341f4612e7c31ed13db17ac241d28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be26723913b8103d1b1fe76b7a053782 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64281370e3144ad3135ec71307459d32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46d00b7b481c9a6d0d295658e9bebd64 |
| publicationDate | 2020-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020157441-A1 |
| titleOfInvention | Method for measuring the modulation of the activation of a g protein-coupled receptor with gtp analogues |
| abstract | The invention concerns a method for determining the ability of a molecule to modulate the activation of a G protein-coupled receptor (GPCR), the method comprising the following steps: a) introducing, into a first container: - a membrane preparation bearing one or more GPCR(s) and one or more Galpha protein(s), - a source of non-hydrolysable or slowly hydrolysable GTP labelled by a first member of a pair of RET partners, - a ligand of the alpha sub-unit of a G protein (Galpha protein) labelled by a second member of the pair of RET partners, the ligand being capable of binding with the full Galpha protein bound to the non-hydrolysable or slowly hydrolysable GTP labelled by the first member of a pair of RET partners, - optionally, a GPCR agonist; b) measuring the RET signal emitted in the first container; c) introducing, (i) into a second container, the same reagents as in step a) and the molecule to be tested or, (ii) into the first container, the molecule to be tested; d) measuring the RET signal emitted in the second container or in the first container obtained in step c); e) comparing the signals obtained in steps b) and d), a modulation in the signal obtained in step d) relative to that obtained in step b) indicating that the molecule to be tested is capable of modulating the activation of the GPCR. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114277072-A |
| priorityDate | 2019-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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