http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020135669-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6d4fb049b25273c568f9c451db6b039e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2019-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b914a9e3fda7a34d9e9a3c745f63800 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ae49cdc8e2283128b3d71b13a4f3a14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99120c6013d73b3d31013b0e3ef6959e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0623e22e02cd17a490b15250a42c52d |
| publicationDate | 2020-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020135669-A1 |
| titleOfInvention | Gene synthesis method |
| abstract | A gene synthesis method. Said method comprises: segmenting multiple target genes into several overlapping oligonucleotide sequences, adding a restriction enzyme cutting site sequence and a tag sequence at the end of the overlapping oligonucleotide sequences, the tag sequence being different due to different target genes; specifically linking the overlapping oligonucleotide sequences in the form of a single-strand and linked with the tag sequence to a magnetic bead of a reverse complement linked with the tag sequence by means of a double-stranded structure, then performing restriction enzyme cutting in a water-in-oil system to obtain single-stranded overlapping oligonucleotides, and then performing polymerase chain assembly, so as to obtain multiple target gene fragments at the same time. |
| priorityDate | 2018-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.