| abstract |
Disclosed are a β-lactoglobulin-vitamin conjugate comprising sugar alcohols and a preparation method thereof. The β-lactoglobulin-vitamin conjugate comprising sugar alcohols comprises β-lactoglobulin that has been subjected to glycosylation treatment with high voltage pulses, VE, sorbitol and maltitol. The glycosylation treatment with high voltage pulses involves treating a 1 mg/mL β-lactoglobulin solution prepared with a 0.01 mol/L, Ph 7.4 phosphate buffer solution in a high voltage electric field for 80 μs, the field intensity of the high voltage electric field being 30 kV/cm, and the electric field waveform being a unipolar square wave. Modification of β-lactoglobulin by the glycosylation treatment with high voltage pulses alters the secondary and tertiary structures of β-lactoglobulin, thus effectively reducing protein sensitization. Further, addition of the sugar alcohols as a natural emulsifier enhances the rate of conjugation of the β-lactoglobulin-vitamin conjugate. |