| abstract |
Methods of treating cells are disclosed. The methods include introducing a media comprising at least about 1 x 10 6 cells/mL into a perfusion chamber having a volume of 50 mL or less, introducing a volume effective to treat the cells of at least one additive selected from cell culture media, a transducing agent, a pH control agent, and a cell activator into the perfusion chamber, and withdrawing cell waste and byproducts from the perfusion chamber, and harvesting the treated cells. The methods may include introducing the media comprising at least about 3 x 10 6 cells/mL into the perfusion chamber. The methods may include measuring and/or controlling at least one parameter of the cells or the media selected from pH, optical density, dissolved oxygen concentration, temperature, and light scattering. |