http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020120747-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d7a89aa6fec722cc898938029782087b |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-501 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-161 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6855 |
filingDate | 2019-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ef4695fef1608711fbb134a67191151 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bec848e1dfe89bf6aeaeb1f0ea8387c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_891bebaecb75ab8489209945522fd51a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf2d08b29e1d55b78a2c3ceea9aed529 |
publicationDate | 2020-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2020120747-A1 |
titleOfInvention | Nucleic acid amplification and identification method |
abstract | The present invention provides a method for generating labelled amplification fragments of a nucleic acid template comprising the steps of providing said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner thereby creating an elongation product, wherein said elongating reaction stops when the elongation product reaches the 5' end of the template nucleic acid or a nucleic acid elongation stopper that is annealed to the template nucleic acid downstream of the elongation product, providing an adaptor nucleic acid that comprises an identification sequence on its 5' end,wherein said identification sequence does not hybridize to the elongation stopper when in con- tact thereto, ligating the adaptor nucleic acid at its 5' end to the 3' end of the elongation product, thereby generating a labelled amplification fragment. |
priorityDate | 2018-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 272.