http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020107495-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dc1fc836ad24085a8aac7c4f451b55cb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-305 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-0065 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-4077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5047 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 |
| filingDate | 2018-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0b6f5939a8ba64e94c944987f83412e |
| publicationDate | 2020-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020107495-A1 |
| titleOfInvention | Preparation method for lymphocyte sample for flow cytometry analysis |
| abstract | The present invention relates to the field of medicines, and provided is a preparation method for a lymphocyte sample for a flow cytometry. The preparation method comprises: centrifuging an anticoagulant blood sample to obtain blood cell sedimentation, diluting by a PBS solution, then centrifuging by adding the blood cell sedimentation into a centrifugal tube containing a lymphocyte separation liquid, and discarding the plasma to obtain buffy coat cells; then adding same into the centrifugal tube, adding the PBS solution, centrifuging and discarding the supernatant, then adding the PBS solution to adjust the concentration of cells, and adding the cell solution into a flow tube; then adding antibodies and mixing fully and evenly, and incubating by avoiding light; centrifuging and discarding the supernatant, and adding the pre-cooled PBS-EDTA solution and then re-suspending the cells to obtain the sample. In the sample obtained according to the method in the present invention, the lymphocytes are enriched and purified, the activity of lymphocytes is well preserved, and granulocytes, erythrocytes, platelets and other blood components are removed as far as possible, so that subgroup grouping that needs to be detected is obviously purified, the analysis and the detection are more accurate, and the costs for the analysis of antibodies and reagents are saved. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113462518-A |
| priorityDate | 2018-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.