| abstract |
Provided are a reference DNA and use thereof, the reference DNA fragment being selected from the group consisting of: (i) DNA fragment 1, characterized by carrying a determined genetic mutation and at least one another artificially altered base X2, wherein compared with a wild type of the gene, at least one determined base X1 in the determined genetic mutation has a mutation related to the occurrence, diagnosis, and/or treatment(for example, a target targeted by a medicament) of a disease (such as a tumor), the mutation is a substitution mutation, a deletion mutation and/or an insertion mutation, and the artificially altered base X2 is distinguished from the determined mutant base X1 related to disease occurrence, diagnosis and/or treatment contained in the DNA of a sample to be detected, (ii) DNA fragment 2, characterized by differing from the DNA fragment 1 merely by not comprising the determined base X1 mutation, or (iii) a mixture of the DNA fragment 1 and the DNA fragment 2. |