| abstract |
Provided is a low-protein serum-free cell culture medium, the culture medium containing a basal physiological buffer mixture and protein components, wherein the protein components comprise bovine serum albumin, transferrin and insulin, and the total addition amount of the protein components does not exceed 30 mg/L. The only three protein components contained in the culture medium are relatively low in content, which is conducive to separating and purifying a product, to improving the quality of the product, to supporting hybridoma cells well, and to increasing the highest cell density and antibody production of hybridoma cells. |