| abstract |
A rapid identification method for fungi in the human body on the basis of Sanger sequencing, comprising a DNA extraction and quality testing step, a primer design and synthesis step, a PCR amplification step, a PCR product purification step, a PCR product purification step, a sequencing step, and a data processing step. The gene sequences of particular species are amplified by using specific primers and are sequenced and aligned for respective identification thereof. |