http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020103192-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a4b7bbde4d87954899cbd3c4939f9d5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0667 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2018-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc97cce63d3be36d05697191a50cf2dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a023cf5734478336ec70ee02d40fdda6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10bd18aa8b2bd2a3fbdfa1e2676fd79a |
| publicationDate | 2020-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020103192-A1 |
| titleOfInvention | Method for extracting exosome |
| abstract | A method for extracting an exosome, comprising the following steps: using a culture medium containing serum free of an exosome to cultivate human adipose-derived mesenchymal stem cells to obtain a culture supernatant containing an exosome; performing a first centrifugation treatment on the culture supernatant, performing a second centrifugation treatment on the supernatant, and then collecting the supernatant to obtain a centrifugate; subjecting the centrifugate to ultrafiltration and concentration treatment using an 100 KD ultrafiltration tube to obtain an ultrafiltrate; subjecting the ultrafiltrate to a third centrifugation treatment, and performing filtration and sterilization treatment using an 0.22 μm filter to obtain a concentrated liquid; and subjecting the concentrated liquid to a fourth centrifugation treatment, and discarding the supernatant, so as to obtain the exosome. |
| priorityDate | 2018-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 85.