| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53c911e75ea09dd4c2705db5306dae77 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827 |
| filingDate |
2019-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc7398e47e2007081a4367d407a956ef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa8f687f73219288c60b637409799831
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_990b7b262053b8526e198ef611842c2a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40082908623359aa22cfa0045076c6a0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a232ac4a6aac1481ff509362154e363 |
| publicationDate |
2020-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2020094457-A1 |
| titleOfInvention |
Liquid sample workflow for nanopore sequencing |
| abstract |
The present invention relates to a method of characterizing a target DNA polynucleotide using rolling circle amplification (RCA) and a synthetic single guide RNA (sgRNA) to identify and cleave the WT version of the target DNA polynucleotide. Also provided are characterization steps based on the use of a transmembrane pore and a DNA translocase enzyme controlling the movement of the DNA polynucleotide through the transmembrane pore. Further envisaged is a kit comprising one or more oligonucleotides specific for at least a portion of the target DNA polynucleotide, an sgRNA specific for the WT version of the target DNA polynucleotide and an sgRNA-guided nucleic acid-binding protein. |
| priorityDate |
2018-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |