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filingDate 2019-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2020-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2020077290-A9
titleOfInvention Analysis of viable and nonviable cells
abstract Provided are methods, compositions, and kits for selectively analyzing a sample for viable cells and nonviable cells. For example, the method may include dyeing viable cells and/or nonviable with a membrane-permeable fluorescent dye, and dyeing nonviable cells with a membrane-impermeable fluorescent quenching dye. The method may include illuminating to cause fluorescent emission from the membrane-permeable fluorescent dye in the viable cells and the membrane-impermeable fluorescent quenching dye in the non-viable cells. The method may include quenching at least a portion of fluorescence of the membrane-permeable fluorescent dye in the nonviable cells by the membrane-impermeable fluorescent quenching dye. The method may include selectively analyzing the sample for the viable and nonviable cells.
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