| abstract |
Provided are an immune composition, a preparation method therefor, and a use thereof. In the present invention, a prokaryotic expression system or a recombinant adenovirus system is used to highly efficiently express VZV envelope gE glycoprotein and the flagellin fusion protein thereof. The produced recombinant gE protein, gE flagellin fusion protein, and recombinant adenovirus vector, or compositions thereof is used to immunize a mouse so as to promote the body to generate high levels of gE and VZV specific antibody titer, as well as gE and VZV specific cell immunity. The present invention has relatively good immunogenicity and can be developed into a new generation of modified VZV vaccines. Unlike the commercial attenuated vaccines currently available, the vaccines developed using the present invention would not make vaccine receiver suffer the risk of contracting Shingles. In addition, the vaccines developed using the present invention have lower side effect than existing subunit Shingles vaccines containing adjuvant gE. |