http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020049274-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c8ed020d9d1eed3acfa2285b2f55de30 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H01J49-165 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2019-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa35d1a7df470128c3b6640b8d822ee0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7b67fc4db61da1cee74c8eea51ac881 |
publicationDate | 2020-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2020049274-A1 |
titleOfInvention | Detection of membrane proteins by mass spectrometry |
abstract | A method of detecting a membrane protein by mass spectrometry comprises: (a) providing a solution comprising a native membrane vesicle having a lipid bilayer to which said membrane protein is attached; (b) sonicating the vesicle in the presence of a mass spectrometry-compatible buffer; (c) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (d) vaporising the sonicated solution using the nanoelectrospray ionisation source under conditions such that the membrane protein is released from the vesicle; (e) ionising the membrane protein; (f) resolving the ionised membrane protein using the mass analyser; and (g) detecting the resolved membrane protein using the detector. Also provided are solutions comprising sonicated vesicles for use in mass spectrometry methods. |
priorityDate | 2018-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 955.