| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7c56973daed8bcc29298b03054ce2b77 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1072
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 |
| filingDate |
2018-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98078469d0344ef6e8feffee0b641465
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb47dfb2b2e1266e1e805f0552bdfffd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3d96ef2a81956b840ee563a4c6c7f3c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d8f2b7abc7dff7c603865193a8e2d7c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de9c3e4d27fe66ca741364c58a8b19ae
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5d8758f32b41af2b64282dd79df74c9 |
| publicationDate |
2020-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2020047769-A1 |
| titleOfInvention |
Method for building rna library and kit |
| abstract |
Provided are a method for building an RNA library and a specialized kit thereof. The method for building the RNA library comprises the following steps: RNA extraction and fragmentation; 3' end tailing; adding a linker to a 5' end and hybridizing the 5' end with a DNA probe mixture; the DNA probe mixture consisting of several DNA probes that are reverse complementary to RNAs that are expected to be removed; removing RNAs from hybrid strands and removing DNAs; and performing reverse transcription and PCR amplification to obtain a library solution. The present invention combines polyA tailing and 5' end ligation. The content of RNAs in the system can be increased rapidly by polyA tailing, thereby avoiding the subsequent purification loss. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112680797-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112176031-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112680797-B |
| priorityDate |
2018-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |