| abstract |
The present invention provides three recombinant E. coli strains for the production of the enzymes CPD-photolyase, 6,4-bifunctional photolyase and 6,4-photolyase, from bacterial Antarctic isolates of the genus Hymenobacter the first one and Sphingomonas the others. Also provides the process of production and purification of the recombinant enzymes with high performance, high degree of purity and high catalytic repair activity, having applications in, but it is not limited to, cosmetics and pharmaceutical industry. Other aspect of this invention is to provide a fast, cheap and qualitative method for the determination of the CPD-photolyase activity. |