http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020022476-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8fa694496041c73b5b38b30db01ba31e |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27bb015bd582a5fb4f826c02a7d1ec74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43b43283206fddf07aa91bb3afbb923d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12c5c47bf69d4f552f3a8886067e089e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03436340ee6f2c2eaee315947ef3ece1 |
| publicationDate | 2020-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2020022476-A1 |
| titleOfInvention | System for highly efficient modification of target sequences |
| abstract | Provided is a technology for more efficient genetic modification, including target gene disruption, using a guide RNA-dependent nuclease. The present invention also addresses the problem of different cleavage efficiencies for each target sequence. Specifically, by the present invention, a new system was developed in which, when developing a target cleavage system that utilizes a guide RNA-dependent nuclease, only the nuclease gene is integrated into a negative-strand RNA viral vector, which has the properties of high infection efficiency and high transgene expression, and, after the viral vector has been introduced into cells in advance and the nuclease has been caused to be expressed, guide RNA is separately supplied to the cells. As a result, an increase in cleavage efficiency and a reduction in recleavage were both successfully achieved, and the efficiency of target gene modification was improved. The present invention is useful as an efficient genetic modification technology, and also has the feature of making it possible to adjust the enhancement and attenuation of genetic modification efficiency by adjusting cleavage efficiency through adjustment of the amount of guide RNA that is introduced. |
| priorityDate | 2018-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 952.