http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019237799-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2ae36d84ea4af395b8f5b7a4d46ec880 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K2211-1044 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K11-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09K11-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2019-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0e2e9ce49fae817be1950821cb879a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbf1a21a633d48c6d48443811df1fa4d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39cad45c7f5b362060f4538bf7d1148b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bdc256110059d69b3e513645a799ef9 |
| publicationDate | 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2019237799-A1 |
| titleOfInvention | Method for detecting choline using cu-nanocluster-complex-based ratiometric fluorescent probe |
| abstract | The present invention belongs to the technical field of the preparation of noble metal nanomaterials and ratiometric fluorescent probes, and in particular relates to a lysozyme-stabilized cu nanocluster complex and a method for preparing a ratiometric fluorescent probe thereof. The cu nanocluster is prepared by using hydrazine hydrate as a reducing agent, and a lysozyme as a stabilizer. A choline oxidase catalyzes choline to produce hydrogen peroxide, and hydrogen peroxide and horseradish peroxidase oxidize o-phenylenediamine to produce diaminophenazine. An inner filter effect occurs between diaminophenazine and the cu nanocluster; with the increase in the concentration of choline, the fluorescence of diaminophenazine gradually increases, whereas the fluorescence of the cu nanocluster is gradually quenched by diaminophenazine. Based on this, a linear relationship between the intensity ratio of the fluorescence peaks of the cu nanocluster and diaminophenazine and the concentration of choline can be constructed, thereby preparing the ratiometric fluorescent probe. The probe is simple in preparation method, low in cost, and high in product sensitivity, and can be developed into a new ratiometric fluorescent probe for use in the highly efficient detection of choline in a biological sample. |
| priorityDate | 2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 89.