http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019237395-A1

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filingDate 2018-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ecf59189d87ad2e75155d9a94f4f389
publicationDate 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2019237395-A1
titleOfInvention Method for targeted knockout of human cd357 gene by applying crispr/cas9 system
abstract Provided is a method for the targeted knockout of a human CD357 gene by applying a CRISPR/Cas9 system. The method comprises: designing a gRNA according to the PAM design principle of gRNA by means of analyzing the specifics of a human CD357 gene sequence, linking the gRNA with a px459 vector to obtain a recombinant vector for the targeted knockout of the human CD357 gene, and using the gRNA in Jurkat cells to guide a CRISPR/Cas9 system to knock out the human CD357 gene.
priorityDate 2018-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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