http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019237375-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cdc65598b1597887a5b7b42b226ac184 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-866 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 |
| filingDate | 2018-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ecf59189d87ad2e75155d9a94f4f389 |
| publicationDate | 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2019237375-A1 |
| titleOfInvention | Method for fixed-site integration of hdmx gene into mcf-7 cells, and application thereof |
| abstract | A method for fixed-site integration of an HDMX gene into MCF-7 cells, using an insect protein expression system to synthesise the necessary elements required for recombinant adeno-associated virus (rAAV), and achieving the objective of fixed-site integration of an HDMX gene into the AAVS1 site of chromosome 19 of MCF-7 cells. The transformed MCF-7 cells stably over-express the HDMX protein, and the necessary elements for rAAV being synthesised from an insect protein expression system avoids the risk of potential endotoxin contamination by an E. coli gene cloning system, enhancing the safety and usability of the MCF-7 cells in preclinical research. |
| priorityDate | 2018-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.