http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019237373-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cdc65598b1597887a5b7b42b226ac184 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2018-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ecf59189d87ad2e75155d9a94f4f389 |
| publicationDate | 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2019237373-A1 |
| titleOfInvention | Method for constructing 293t cell strain with site-directed insertion of btdc gene and use thereof |
| abstract | Disclosed are a method for constructing a 293T cell strain with site-directed insertion of the BTDC gene and a use thereof. The cell strain is obtained by constructing an sgRNA expression vector based on a CRISPR/Cas9 system according to the nucleotide sequence of an insertion site; constructing, according to an sgRNA action site, an expression cassette that contains the BTDC gene and can be integrated into a host genome; then co-transfecting the constructed sgRNA expression vector and a linearized expression cassette into 293T cells; and screening to obtain the 293T cell strain with site-directed insertion of the BTDC gene. The obtained cell strain can maintain the efficient and stable expression of the BTDC protein for a long period of time. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951782-A |
| priorityDate | 2018-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.