http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019228542-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5bd1a176ef2d8d39b992712a7df6743 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 |
| filingDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a14c964b28ea9ca0727975f79a2db2c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c2c969a91a2f43d743cb9b563eee35a |
| publicationDate | 2019-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2019228542-A1 |
| titleOfInvention | Primer 5' end reverse complementary fluorescent pcr and test kit |
| abstract | Disclosed is a primer 5' end reverse complementary fluorescent PCR, wherein a primer 5' end pair is added to a sequence for reverse complementary fluorescent PCR amplification, amplification product ends are complementary, and target product 3' ends may also be mutual templates and mutual primers to increase amplification efficiency. The sensitivity is increased by 50 times on the basis of a conventional PCR index amplification of 109-12, or 5 fewer amplification cycles are performed under same sensitivity conditions. In addition, the primer 5' end complementarity does not extend but rather inhibits original primer pair 3' end polymerisation non-specificity, or the 5 fewer amplification cycles avoid primer non-specific amplification regions. An amplification product of primers having central portion same sequences and 3' final 1-2 base adenine ends + UDG enzymolysis with dU instead of dT in combination with mineral oil sealing causes no primer dimer (PD) production within PCR reaction cycles and prevents product aerosol cross-contamination. |
| priorityDate | 2018-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 115.