http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019214063-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a9d416d7f82980ff7380f92b3c6ea61d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-09 |
| filingDate | 2018-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_faddd81f27d26fb982a3fc4abda2d60c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9138f9953d5c93121a431f8f46174c64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_910956cd08d636e8da06a420048d4553 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7387da2c771270e51ce52820d1f9af49 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d6e867825479406232326ee31cf349d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7535776dad323d3500e3285aee831cef |
| publicationDate | 2019-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2019214063-A1 |
| titleOfInvention | Method for detecting circulating tumor cells |
| abstract | A method for detecting circulating tumor cells, comprising: collecting a human body fluid sample and fixing same with formaldehyde to improve the cell membrane permeability; adding cells to a plurality groups of PCR multi-tube strips, a reverse transcription primer carrying ID1 being added to each strip; after the cells are reverse-transcribed into cDNA molecules, collecting the cells, mixing same, and adding same to another group of PCR multi-tube strips, an extension primer carrying ID2 and having 3' terminus capable of specifically identifying 3' terminus of a cDNA molecule being added to each strip; performing an extension reaction after sequence complementation; collecting and lysing the cells; and pre-proliferating the extension product using a PCR primer. High-throughput sequencing is performed to analyze the combination of ID1:ID2. In addition, the reverse transcription and PCR proliferation for different markers can accurately identify circulating tumor cells and circulating tumor cells in which EMT occurs, and can be used in combination with other technologies to detect extremely few circulating tumor cells and other non-body fluid rare cells in a body fluid sample to the maximum extent. |
| priorityDate | 2018-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 225.