http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019205919-A1

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filingDate 2019-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ef444c0d53e00e34f6c8e37e283cee1
publicationDate 2019-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2019205919-A1
titleOfInvention Construction method for biological mutant library
abstract Disclosed in the present invention is a construction method for a biological mutant library. The construction method for a biological mutant library disclosed in the present invention comprises: 1) introducing a DNA segment to a target organism to obtain a genetically modified organism, the DNA segment being capable of expressing elements required by implementation of site-directed mutation of target DNA in the genetically modified organism; and 2) culturing the genetically modified organism to obtain a biological mutant library, the organism being a sexual reproductive organism, and the genetically modified organism being capable of site-directed mutation of target DNA in a germ cell. According to the mutant library obtained by using the present method, organisms having the characteristics of anti-biotic stress, anti-abiotic stress, high yield, good quality characteristics, high secondary metabolite yield, etc. can be obtained by screening. Therefore, the present method has a great application prospect.
priorityDate 2018-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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