Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-179 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-631 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-48721 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-487 |
filingDate |
2019-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_266ef822253c28bf616a93fe1c86565d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a59c96a29fc0bfd7072670a952ecefd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2849c5fc8ba7674fa75bcca711af692c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0aacd62cbe1a25f44ba461723eb82b47 |
publicationDate |
2019-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2019204720-A1 |
titleOfInvention |
Nucleic acid sequencing methods and computer-readable media for practicing same |
abstract |
Provided are methods of nucleic acid sequencing. The methods include producing a circularized DNA including a full-length cDNA and a known heterologous sequence, and performing rolling circle amplification using the circularized DNA as template to produce a concatemer including repeating segments including the full-length cDNA and the known heterologous sequence. The methods further include obtaining a raw sequencing read of the concatemer using a nanopore, identifying the repeating segments in the raw sequencing read, and producing a consensus sequence of the full-length cDNA based on the sequences of the repeating segments. Computer-readable media, computing devices, and systems that find use, e.g., in practicing the methods of the present disclosure are also provided. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021127406-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022240764-A1 |
priorityDate |
2018-04-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |