abstract |
The invention relates to the food industry and to medicine and concerns methods for obtaining transformed plant cells containing human recombinant alkaline phosphatase. The present method includes creating a plant expression vector containing a human alkaline phosphatase gene, integrating the plant expression vector containing a human alkaline phosphatase gene into Agrobacterium strains, obtaining callus plant cells, carrying out the Agrobacetrium transformation of said callus cells using the Agrobacterium strains, obtaining somatic embryoids from the transformed callus cells, and cultivating somatic embryoids containing the human alkaline phosphatase gene in a suspension culture. The purpose of the invention is to regulate the microflora of the gastrointestinal tract and to protect and restore the body's immune system. |