http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019144660-A1

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publicationDate 2019-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2019144660-A1
titleOfInvention Yeast genome editing vector, construction method therefor and application thereof
abstract A yeast genome editing vector, a construction method therefor and an application thereof: a guide RNA (gRNA) coding plasmid P is used as a starting vector, 34-bp LoxP sequences of the same direction are introduced at two ends of a replication origin point Ori of the vector, and a Cre recombinase gene expression cassette is introduced to a backbone position of the plasmid P, thus the yeast genome editing vector is obtained. An application of the vector in gene editing that uses CRISPR/Cas9 as a medium, which enables multiple rounds of editing to be carried out on the Saccharomyces cerevisiae genome.
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