http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019136169-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27e1ea5f65e8f958e48f1a9d53fdbb45 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1068 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2019-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6703040485afca166ab0a669dcf52090 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ebdaf1d97f75cd3ea096266aa5b94fd |
publicationDate | 2019-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2019136169-A1 |
titleOfInvention | Versatile amplicon single-cell droplet sequencing-based shotgun screening platform to accelerate functional genomics |
abstract | Disclosed is a method of functional genomics determination including transducing a cell population with a set of nucleic acid molecules including a pooled library of genomic perturbagens to integrate multiple perturbagen cassettes into the genome. A phenotype of individual cells is determined and single cells of the population with targeted phenotypes are individually sorted into a set of compartments. Each compartment includes a forward primer with a nucleic acid sequence (NAS) that specifically binds a common nucleic acid sequence on the nucleic acid molecules and a compartment (cell)-specific nucleic acid barcode. Also included is a reverse primer with a NAS that specifically binds a common NAS on the nucleic acid molecules comprising a pooled library of genomic perturbagens. The genome-integrated perturbagen cassettes are create amplicons which are pooled and sequences determined. This method can be applied to other genome-level single-cell applications - immune receptor profiling, targeted DNA/RNA sequencing, and metagenomics. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10802026-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10787710-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11124791-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11525831-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10618932-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10648978-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11624747-B2 |
priorityDate | 2018-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 223.