http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019136034-A1

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filingDate 2018-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee6cc758037529f0d648eb3d6d1157b8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12037778950a81aafe21d4590cf5e205
publicationDate 2019-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2019136034-A1
titleOfInvention Loop-mediated isothermal amplification (lamp) based assay for detecting microbes
abstract A method and system are disclosed for detecting microbial pathogens in a sample suspected of containing the pathogens. The method includes combining loop-mediated isothermal amplification (LAMP) reagents and a polymer gel, such as a hydrogel, together with the sample to form a mixture. The gel polymerizes over a short time to immobilize the viral particles within the mixture. If target DNA/RNA are present in the sample, amplicons are produced. The target microorganisms are detected by visually detecting the presence or absence of the amplicons. The target microorganism concentrations may be determined based on the number of fluorescent amplicon dots after the reaction using a smartphone or a fluorescent microscope. The method may be employed for rapidly and inexpensively quantifying microbial pathogens in environmental water samples with high sensitivity.
priorityDate 2018-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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