Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2b8be16808b103661c35ab01e58412a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3244ba8390b1f290f4ba36389c82488b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_66825b3e343b7f38f92fe11cf467e95c |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1072 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1034 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
2018-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67f85a8b11022cfbd1fe3a317ca8a36d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_799b1f86bd1bb6976409fa1cb0d1c6c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4864d779ffefffe2e8f45b952e22139f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11f9e73268c149173db7ac9397c7fa71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df78390cd854b81ad147166a2b69c489 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42ac3095a339b36264cb8033bed9d170 |
publicationDate |
2019-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2019121842-A1 |
titleOfInvention |
Target enrichment by unidirectional dual probe primer extension |
abstract |
The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The 5 hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is 10 extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022194764-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021191276-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022008578-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021053008-A1 |
priorityDate |
2017-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |