| abstract |
A test kit for 1,5-anhydro-D-glucitol (1,5-AG) and a detection method thereof. The test kit can implement an energy supply of a sample in a reaction cycle process, eliminate the interference of glucose and test byproducts of glucose 6-phosphate and pyruvic acid in the sample, and avoid a reverse reaction caused by the product accumulation. After the glucose and the byproduct accumulation are eliminated, 1,5-AG is converted into 1,5-anhydro-fructose and hydrogen peroxide. Hydrogen peroxide reacts with a chemiluminescent substrate to form a continuously stable chemiluminescence signal. Compared with the traditional enzymatic colorimetric method, the test kit has advantages of high sensitivity, high accuracy, high specificity, strong anti-interference, good stability, etc. |