| abstract |
The present invention relates to silica nanoparticles for biomarker diagnosis and a method for producing the same and, more particularly, to silica nanoparticles for biomarker diagnosis capable of measuring glycated proteins such as glycated hemoglobin and glycated albumin used as marker substances for diagnosing diabetes, and a method for producing the same. Silica nanoparticles for biomarker diagnosis, according to the present invention, in which reactive polymers and non-reactive polymers are immobilized on the surface thereof and dye is immobilized in the inside thereof, immobilize the dye inside the silica nanoparticles by means of a covalent bond, and thus have advantages in that the dye is not affected by external environments such as heat and pH, and stability is enhanced even in the case of long-term storage. Also, as multiple dye molecules are immobilized in one silica nanoparticle, the quantity of light absorption that one particle has is amplified compared to the absorption quantity of one dye molecule such that it is possible to accurately measure the quantity of glycated proteins having a low detection limit. In addition, since reactive polymers for increasing dispersion stability, obtained by combining non-reactive polymers and probes, are immobilized on the surface of the silica nanoparticles in which the dye is immobilized, influence due to various proteins and salts included in a sample is minimized, and thus the silica nanoparticles can be utilized to rapidly and accurately measure a biomarker to be detected by using a stable method by applying the silica nanoparticles in a point-of-care testing system using an actual bio-sample. |