http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019114157-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-13142 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P33-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0073 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P33-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2018-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ad074ee989f0a1515ee93a9e23fc911 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f495cdbd0abdd26a3bb5a9157483a6c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dabe7ccf9a9ecc655b60dff821501707 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff5c3e4334985487ea70418c7386cd25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_934d151d23b1b5e11e2f565e9b5705e5 |
| publicationDate | 2019-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2019114157-A1 |
| titleOfInvention | METHOD FOR ENZYMATICALLY CONVERTING AND PRODUCING 9α-HYDROXYANDROST-4-ENE-3,17-DIONE |
| abstract | Provided is a method for enzymatically converting and producing 9α-hydroxyandrost-4-ene-3,17-dione, the method comprising the steps of: expressing, in E. coli BL21, an oxidative subunit KshA, a reductive subunit KshB, and a subunit KshC, which has an unknown activity, of 3-ketosteroid-9α-hydroxylase derived from Mycobacterium sp. strain VKMAc-1817D, and identifying KshC as an oxidative subunit with a much higher enzymatic activity than KshA; expressing a formate dehydrogenase FDH by using BL21/pET-28a(+)-fdh constructed in the present laboratory, by using KSH (KshB+KshC) and the crude enzyme solution of FDH engineering strains as a biocatalyst, by using a steroid AD as a substrate and at a reaction temperature of 30℃ and a pH of 7.0; and converting AD under optimal conditions to generate the product 9-OH-AD, wherein within 20 hours, the yield of 9-OH-AD is 4.7 g/L and the molar conversion rate reaches 96.7%. |
| priorityDate | 2017-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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