| abstract |
The present invention relates to a genome editing composition using a CRISPR/Cpf1 system and a use thereof and, more particularly, to a genome editing composition comprising: a CRISPR RNA (crRNA) including a guide sequence capable of hybridizing with a target nucleotide sequence, and a uridine repeat sequence connected to the 3'-terminus of the guide sequence, or a DNA coding therefor; and a Cpf1 protein or a DNA coding therefor, a genome editing method using the same, a method for construction of a transgenic entity, and a transgenic entity. Utilizing the CRIPSPR/Cpf1 system, the present invention can increase an indel efficiency and decrease off-target activity in genome editing and thus can easily construct a transformed cell or transgenic animal or plant having a desired gene inserted thereinto (knock-in) or deleted therefrom (knock-out). |