| abstract |
Methods of measuring multiple enzyme activities in parallel in a sequencing-based assay to characterize enzyme activities in individual mammalian cells. In preferred implementations, the methods involve forming microfluidic droplets containing oligonucleotide functionalized microbeads and single mammalian cells, lysing the cells, and allowing enzyme activity on enzyme substrates present in the oligonucleotides, isolating the individual microbeads, and determining the enzymatic activity to quantitate and evaluate the enzymatic activity or capacity of the cells. |